Description
Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-OPG polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-OPG polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the OPG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of OPG can be calculated.
Background: Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF), or tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a protein that in humans is encoded by the TNFRSF11B gene. It is a member of the tumor necrosis factor (TNF) receptor superfamily, and comprising 401 amino acid residues arranged into 7 structural domains. It is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). OPG can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors. Elevated OPG levels has been reported in heart diseases, in Placebo effect serum responses in IBS patients and in severe mental disorders